Fig 7

(A) Role of tagln2 in CV pruning in zebrafish embryos. Boxes show enlarged images of the CV. The arrowhead indicates unpruned vessel. Scale bar: 50 μm. (B) Quantification of vascular loops in sibling and tagln2^-/- embryos: sibling, n = 25 embryos; tagln2^-/-, n = 23 embryos. P = 0.0004. Student’s unpaired two-tailed t test. (C) Time-lapse live imaging of Tg(fli1a:EGFP;kdrl:mCherry) embryos shows EC nucleus migration in CV pruning in control or tagln2 morphant. Arrows indicate the direction of the blood flow. Colored circles indicate the EC nuclei. Scale bar: 10 μm. (D) Direction of EC nucleus migration in the regressing vessel. The direction is classified into three types: with the flow, static, and against the flow. A total of 12 vascular loops with 24 EC nuclei and 8 vascular loops with 15 EC nuclei were calculated in the control group and tagln2 morphant, respectively. Unpaired two-tailed chi-square test. P = 0.0471. (E) and (F) Efficiency of siTAGLN2 in HUVECs at the mRNA (P = 0.0094) and protein (P < 0.0001) levels. (G) Wound healing of siCTR-transfected and siTAGLN2-transfected HUVECs. Statistics for wound healing after siCTR and siTAGLN2 transfection: 6 h, P = 0.0037; 12 h, P = 0.0252. (E)-(G) Student’s unpaired two-tailed t test. *P < 0.05, **P < 0.01, ***P < 0.001.