IMAGE

Fig 5

ID

ZDB-IMAGE-210802-19

Genes

EGFP
mRFP

Publication

Wen et al., 2021 - The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics

All Figures

Figures for Wen et al., 2021

Image

Figure Caption

Fig 5 Knockdown of <italic toggle='yes'>klf6a</italic> impairs junction remodeling and rearrangement of actin cytoskeleton in CV pruning.

(A) and (B) Time-lapse live imaging of junction remodeling and actin cytoskeleton dynamics in CV pruning in Tg(Fliep:Lifeact-EGFP);KI(cdh5-mRFP) embryos. (A) Control embryo shows a retraction of junction (white arrowhead) and a shrinkage of its junctional ring (white and blue arrowheads) as multicellular tube became stenotic. F-actin forms at cdh5-positive junction (white, blue and yellow arrowheads), and undergoes a similar rearrangement as cdh5-positve junctions. 7 vascular loops are taken time-lapse live imaging. Scale bar: 25 μm. (B) Although klf6a morphant show F-actin co-localization with junction, however, neither junction nor F-actin does not go through remodeling (arrowhead). 5 vascular loops are taken time-lapse live imaging. Scale bar: 25 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.