FIGURE

Figure 5

ID

ZDB-FIG-210719-5

Publication

Adams et al., 2021 - A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing

Other Figures

All Figure Page

Back to All Figure Page

Figure 5

Mutational analysis of zGDNF and zGFRα1 site 1 and 2 interactions with zRET^ECD

(A) Heatmap of the sequence conservation between hGFRα paralogs, and zGFRα1a mapped onto the structure of zGFRα1a D2-D3 domains reported here. Residues are colored by similarity (red highly similar to yellow through to white, least similar). Two orthogonal views are shown. Right panel, close-up of site 1 and conserved zGFRα1a residues.

(B) Binding curves and K_D values obtained using MST for zGFRα1a^D1-3 and mutations assessed in complex with zGDNF^mat., with a minimum of n = 3 repeats for the WT and the mutations with the SE represented.

(C) Heatmap of the sequence similarity between GDNF paralogs depicted as a surface representation, mapped onto zGDNF^138-235. Right panel, close-up of site 2 contact between RET^CRD and zGDNF dimer.

(D) MST binding curves and K_D values for zGDNF and mutations L156A and Y158A probed in complex with WT zGFRα1a binding to zRET^ECD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Structure (London, England : 1993), 29(7), Adams, S.E., Purkiss, A.G., Knowles, P.P., Nans, A., Briggs, D.C., Borg, A., Earl, C.P., Goodman, K.M., Nawrotek, A., Borg, A.J., McIntosh, P.B., Houghton, F.M., Kjær, S., McDonald, N.Q., A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing, 694-708.e7, Copyright (2021) with permission from Elsevier. Full text @ Structure