FIGURE

Figure 3

ID
ZDB-FIG-210719-3
Publication
Adams et al., 2021 - A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing
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Figure 3

Ligand-co-receptor-induced conformational changes in zRETECD

(A) The CLD3-β2-β3 loop is shown in yellow as sticks (i) projects “downward” in the view shown for zRET CLD(1-4) (see the orientation of Y292 side chain) and (ii) projects “upward” to engage the GFRα1D2 α1 helix (green sticks) in the zRGα1a structure.

(B) The shorter CLD3-β2-β3 loop and extra helix from the human RETECD-NRTN-GFRα2 structure (PDB: 6Q2O) shown as olive colored sticks, domains colored as in Figure 1.

(C) Sequence alignment of RET CLD3-β2-β3 loop sequences by Espript (http://espript.ibcp.fr) (Robert and Gouet, 2014).

(D) Binding curves and calculated KD values for zRETECDwt and mutant (zRETECDP291-Q296:AAG) binding to zGFRα1a2-zGDNF2 measured by MST, with a minimum of n = 3 repeats for the WT and the mutations with the SE represented.

(E) (i) Electron density map calculated using m2Fo-DFc coefficients over the CLD3-β2-β3 loop, yellow sticks and contoured at 1.0σ. (ii) Coulombic potential cryo-EM map for CLD3-β2-β3 loop from the zRGα1a complex (black mesh). Calcium ions are represented as pale green spheres.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Structure (London, England : 1993), 29(7), Adams, S.E., Purkiss, A.G., Knowles, P.P., Nans, A., Briggs, D.C., Borg, A., Earl, C.P., Goodman, K.M., Nawrotek, A., Borg, A.J., McIntosh, P.B., Houghton, F.M., Kjær, S., McDonald, N.Q., A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing, 694-708.e7, Copyright (2021) with permission from Elsevier. Full text @ Structure