FIGURE

Figure 4

ID

ZDB-FIG-210719-4

Publication

Adams et al., 2021 - A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing

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Figure 4

Different GFL ligands establish a conserved spacing between RET CRD-CRD pairs in their respective ternary complexes

(A) Separation between the Cα of E613 (equivalent to E620 of hRET) from both molecules of zRET^ECD within the zRGα1a structure.

(B) Equivalent distance between the Cα E620 from both molecules of hRET^ECD from the hRET^ECD-NRTN-GFRα2 (PDB: 6Q2O) structure.

(C) Equivalent separation between the Cα E620 from the two molecules of hRET^ECD from the hRET^ECD-GDF15-GFRAL (PDB: 6Q2J) structure. The overall structure is represented as a cartoon and the Ca²⁺ ions are represented as spheres. RET is colored cyan, teal, and pale cyan in zRGα1a, hRET^ECD-NRTN-GFRα2, and hRET^ECD-GDF15-GFRAL structures, respectively. GFRα1a, GFRα2, and GFRAL are colored green, dark green, and pale green, respectively. GDNF, NRTN, and GDF15 are colored orange, red, and light pink, respectively.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Structure (London, England : 1993), 29(7), Adams, S.E., Purkiss, A.G., Knowles, P.P., Nans, A., Briggs, D.C., Borg, A., Earl, C.P., Goodman, K.M., Nawrotek, A., Borg, A.J., McIntosh, P.B., Houghton, F.M., Kjær, S., McDonald, N.Q., A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing, 694-708.e7, Copyright (2021) with permission from Elsevier. Full text @ Structure