Figure 2

Cryo-EM structure of the zRET^ECD-zGFRα1a^D1-3-zGDNF^mat. (zRGα1a) complex

(A) Schematic of zRET^ECD, zGFRα1a^D1-3, and zGDNF^mat., color coded according to Figure 1A.

(B) Orthogonal views of the reconstituted zRGα1a complex cryo-EM map, projecting down the approximate molecular dyad or perpendicular to it. The cryo-EM map is segmented and colored by protein, with zRET^ECD cyan, zGFRα1a^D1-3 green, and zGDNF^mat. orange.

(C) Symmetry-expanded map of zRGα1a half-complex, with the map segmented and colored by protein as in (B).

(D) The final model of the zRGα1a complex built into the C2 symmetry map, colored light gray. The domains are colored as in Figure 1 with zRET^ECD: for GFRα1a domains D1-3 are pale green, green, and dark green, respectively; the two molecules of zGDNF^mat. are orange and pale orange. Two sites of interaction within the zRGα1a complex are highlighted in red dashed boxes, labeled as site 1 (zGFRα1a-zRET) and site 2 (zGDNF-zRET). Interaction residues are highlighted as sticks and the backbone represented as a cartoon.