ZFIN Image: Adams et al., 2021, Figure 4

Image

Figure Caption

Figure 4

Different GFL ligands establish a conserved spacing between RET CRD-CRD pairs in their respective ternary complexes

(A) Separation between the Cα of E613 (equivalent to E620 of hRET) from both molecules of zRET^ECD within the zRGα1a structure.

(B) Equivalent distance between the Cα E620 from both molecules of hRET^ECD from the hRET^ECD-NRTN-GFRα2 (PDB: 6Q2O) structure.

(C) Equivalent separation between the Cα E620 from the two molecules of hRET^ECD from the hRET^ECD-GDF15-GFRAL (PDB: 6Q2J) structure. The overall structure is represented as a cartoon and the Ca²⁺ ions are represented as spheres. RET is colored cyan, teal, and pale cyan in zRGα1a, hRET^ECD-NRTN-GFRα2, and hRET^ECD-GDF15-GFRAL structures, respectively. GFRα1a, GFRα2, and GFRAL are colored green, dark green, and pale green, respectively. GDNF, NRTN, and GDF15 are colored orange, red, and light pink, respectively.

Acknowledgments

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Reprinted from Structure (London, England : 1993), 29(7), Adams, S.E., Purkiss, A.G., Knowles, P.P., Nans, A., Briggs, D.C., Borg, A., Earl, C.P., Goodman, K.M., Nawrotek, A., Borg, A.J., McIntosh, P.B., Houghton, F.M., Kjær, S., McDonald, N.Q., A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing, 694-708.e7, Copyright (2021) with permission from Elsevier. Full text @ Structure