IMAGE

Figure 4

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ZDB-IMAGE-210719-4
Source
Figures for Adams et al., 2021
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Figure Caption

Figure 4

Different GFL ligands establish a conserved spacing between RET CRD-CRD pairs in their respective ternary complexes

(A) Separation between the Cα of E613 (equivalent to E620 of hRET) from both molecules of zRETECD within the zRGα1a structure.

(B) Equivalent distance between the Cα E620 from both molecules of hRETECD from the hRETECD-NRTN-GFRα2 (PDB: 6Q2O) structure.

(C) Equivalent separation between the Cα E620 from the two molecules of hRETECD from the hRETECD-GDF15-GFRAL (PDB: 6Q2J) structure. The overall structure is represented as a cartoon and the Ca2+ ions are represented as spheres. RET is colored cyan, teal, and pale cyan in zRGα1a, hRETECD-NRTN-GFRα2, and hRETECD-GDF15-GFRAL structures, respectively. GFRα1a, GFRα2, and GFRAL are colored green, dark green, and pale green, respectively. GDNF, NRTN, and GDF15 are colored orange, red, and light pink, respectively.

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Reprinted from Structure (London, England : 1993), 29(7), Adams, S.E., Purkiss, A.G., Knowles, P.P., Nans, A., Briggs, D.C., Borg, A., Earl, C.P., Goodman, K.M., Nawrotek, A., Borg, A.J., McIntosh, P.B., Houghton, F.M., Kjær, S., McDonald, N.Q., A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing, 694-708.e7, Copyright (2021) with permission from Elsevier. Full text @ Structure