IMAGE

Figure 2

ID
ZDB-IMAGE-210719-2
Source
Figures for Adams et al., 2021
Image
Figure Caption

Figure 2

Cryo-EM structure of the zRETECD-zGFRα1aD1-3-zGDNFmat. (zRGα1a) complex

(A) Schematic of zRETECD, zGFRα1aD1-3, and zGDNFmat., color coded according to Figure 1A.

(B) Orthogonal views of the reconstituted zRGα1a complex cryo-EM map, projecting down the approximate molecular dyad or perpendicular to it. The cryo-EM map is segmented and colored by protein, with zRETECD cyan, zGFRα1aD1-3 green, and zGDNFmat. orange.

(C) Symmetry-expanded map of zRGα1a half-complex, with the map segmented and colored by protein as in (B).

(D) The final model of the zRGα1a complex built into the C2 symmetry map, colored light gray. The domains are colored as in Figure 1 with zRETECD: for GFRα1a domains D1-3 are pale green, green, and dark green, respectively; the two molecules of zGDNFmat. are orange and pale orange. Two sites of interaction within the zRGα1a complex are highlighted in red dashed boxes, labeled as site 1 (zGFRα1a-zRET) and site 2 (zGDNF-zRET). Interaction residues are highlighted as sticks and the backbone represented as a cartoon.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Structure (London, England : 1993), 29(7), Adams, S.E., Purkiss, A.G., Knowles, P.P., Nans, A., Briggs, D.C., Borg, A., Earl, C.P., Goodman, K.M., Nawrotek, A., Borg, A.J., McIntosh, P.B., Houghton, F.M., Kjær, S., McDonald, N.Q., A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing, 694-708.e7, Copyright (2021) with permission from Elsevier. Full text @ Structure