FIGURE

Figure 7.

ID

ZDB-FIG-200829-70

Publication

Liu et al., 2020 - TMEM216 Deletion Causes Mislocalization of Cone Opsin and Rhodopsin and Photoreceptor Degeneration in Zebrafish

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Figure 7.

TUNEL-positive photoreceptor nuclei were increased in tmem216 knockouts. Cryosections from 7-dpf zebrafish were stained by TUNEL assay (green) and anti-CHOP (Proteintech), counter stained with DAPI. (A) Wildtype. (B, C) tmem216^snyR8Δ60 homozygous retina. Note TUNEL-positive DAPI-labeled rod (arrow in B) and cone (arrow in C) nuclei. (D) Quantification of TUNEL-positive nuclei. Number of TUNEL-positive nuclei were significantly increased in the outer nuclear layer of tmem216^snyR8Δ60 homozygous animals (n = 3, Student's t-test); similar phenotype was observed in tmem216^snyΔ175 animals. (E, F) CHOP staining of wildtype and knockout retina. Immunoreactivity of CHOP antibody was similar between wildtype and tmem216^snyR8Δ60 homozygous retina. Scale bar in A: 6 µm for A, E, and F; 8 µm for B and C.

Expression Data

Antibody:	Ab4-ddit3
Fish:	AB/TU tmem216^{zf3394/zf3394} (AB/TU) tmem216^{zf3395/zf3395} (AB/TU)
Anatomical Terms:	retinal inner nuclear layer retinal cone cell retinal inner nuclear layer retinal rod cell retinal outer nuclear layer retinal cone cell retinal outer nuclear layer retinal rod cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tmem216^{zf3394/zf3394} (AB/TU) tmem216^{zf3395/zf3395} (AB/TU)
Observed In:	retinal inner nuclear layer apoptotic process retinal outer nuclear layer apoptotic process
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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