IMAGE

Figure 7.

ID

ZDB-IMAGE-200829-71

Antibodies

Ab4-ddit3

Publication

Liu et al., 2020 - TMEM216 Deletion Causes Mislocalization of Cone Opsin and Rhodopsin and Photoreceptor Degeneration in Zebrafish

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Figures for Liu et al., 2020

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Figure Caption

Figure 7.

TUNEL-positive photoreceptor nuclei were increased in tmem216 knockouts. Cryosections from 7-dpf zebrafish were stained by TUNEL assay (green) and anti-CHOP (Proteintech), counter stained with DAPI. (A) Wildtype. (B, C) tmem216^snyR8Δ60 homozygous retina. Note TUNEL-positive DAPI-labeled rod (arrow in B) and cone (arrow in C) nuclei. (D) Quantification of TUNEL-positive nuclei. Number of TUNEL-positive nuclei were significantly increased in the outer nuclear layer of tmem216^snyR8Δ60 homozygous animals (n = 3, Student's t-test); similar phenotype was observed in tmem216^snyΔ175 animals. (E, F) CHOP staining of wildtype and knockout retina. Immunoreactivity of CHOP antibody was similar between wildtype and tmem216^snyR8Δ60 homozygous retina. Scale bar in A: 6 µm for A, E, and F; 8 µm for B and C.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.