FIGURE

Figure 2

ID

ZDB-FIG-200627-2

Publication

Weaver et al., 2020 - Hyaloid vasculature and mmp2 activity play a role during optic fissure fusion in zebrafish

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Figure 2

Pax2a is necessary for recruitment of hyaloid vasculature into the optic fissure. (A)In vivo 4D confocal imaging of Tg[kdrl:mCherry] pax2a^+/+,+/− or pax2a^−/− embryos. Time lapse series depicting the region of the OF (dotted white lines) and mCherry positive vasculature endothelial cells migrating through the fissure from 24-29 hpf. Scale bar = 10 μm. (B) Comparison of pax2a^+/+,+/− and pax2a^−/− vascularization during OF fusion, 24-48 hpf. 3D reconstructions of whole mount Tg[kdrl:mCherry] (red) pax2a^+/+,+/− or pax2a^−/− embryos with DAPI (blue) stained DNA. Scale bar = 50 μm. (C) Quantification of the number of mCherry positive cells from 3D confocal stacks within the region of the OF. Individual embryo results are depicted. ANOVA p < 0.0001. (D) Whole mount in situ hybridization comparing tln1 expression between pax2a^+/+,+/− and pax2a^−/− embryos at 28 and 32 hpf. tln1 signal within the OF (yellow arrowhead) appears reduced in Pax2a^−/− embryos (red *). (E) qPCR analysis of tln1 expression from heads of pax2a^+/+ and pax2a^−/− embryos at 32 hpf confirms a reduction in tln1 expression.

Expression Data

Genes:	cldn5b flt4 tjp1a tln1
Fish:	WT pax2a^tu29a/tu29a
Anatomical Terms:	head midbrain hindbrain boundary optic fissure
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax2a^tu29a/tu29a pax2a^tu29a/tu29a; is5Tg
Observed In:	head hyaloid vessel vascular associated smooth muscle cell lens hyaloid vessel midbrain hindbrain boundary optic fissure optic furrow vascular associated smooth muscle cell retina vascular associated smooth muscle cell (all 7)
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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