FIGURE

Figure 1

ID

ZDB-FIG-200627-1

Publication

Weaver et al., 2020 - Hyaloid vasculature and mmp2 activity play a role during optic fissure fusion in zebrafish

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Figure 1

An increase in F-actin dynamics preceding laminin degradation during optic fissure fusion is disrupted in pax2a^−/− embryos. (A) Whole mount immunohistochemistry was used to simultaneously visualize F-actin (red) and laminin (green) during OF fusion, 24-72 hpf. Central-proximal sections obtained using confocal imaging were collected and quantified. Scale bar = 50 μm. (B) Whole mount Immunohistochemistry was used to simultaneously visualize F-actin (red) and laminin (green) during OF fusion, 24-72 hpf, in pax2^−/− embryos. Central-proximal regions of the OF are displayed. Scale bar = 50 μm. (C) Quantification of laminin signal intensity within the OF, normalized to regions of laminin staining juxtaposed to the lens. Relative pixel intensities are displayed. ANOVA p < 0.0001. (D) Quantification of F-actin intensity (phalloidin staining) within the OF, normalized to regions of F-actin signal within the lobe of the retina. Relative pixel intensities are displayed. ANOVA p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax2a^tu29a/tu29a
Observed In:	optic furrow basement membrane optic furrow filamentous actin optic furrow laminin complex
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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