FIGURE

Fig. 6

ID

ZDB-FIG-200614-8

Publication

Castro et al., 2020 - Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain

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Fig. 6

Over expression of HCFC1 decreases NPC number and differentiation. aTg(hsp701: HCFC1) were heat shocked according to the methods section and total RNA was isolated from control (No heat shock (NHS)) and heat shock larvae (HS). Quantitative PCR was performed to test the expression of sox2, elavl3, olig2, and gfap. Error bars represent standard error of the mean. N = 28/group/biological replicate. *p < 0.05. b The total number of Sox2 cells was quantified across forebrain (^#p = 4.88568E−05), midbrain (^##p = 0.004359), and hindbrain (^###p = 0.077). Error bars represent standard error of the mean. N = 5/group. c, c′ Representative images of 2 days post fertilization (DPF) Tg(hsp701:HCFC1) larvae (NHS or HS) stained with anti-Sox2 antibodies. Schematic demonstrates 2 DPF larval brain section with graphical inset of hypothalamus region (Hy) to specify region shown

Expression Data

Genes:	elavl3 gfap olig2 sox2
Fish:	ute1Tg
Condition:	heat shock
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ute1Tg
Condition:	heat shock
Observed In:	forebrain neuronal stem cell hindbrain neuronal stem cell midbrain neuronal stem cell whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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