FIGURE

Fig. 5

ID

ZDB-FIG-200614-7

Publication

Castro et al., 2020 - Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain

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Fig. 5

The hcfc1a^co60/+ allele increases the expression of differentiation markers. a, a′, b and b′ Representative images of brain sections from Tg(gfap:EGFP) larvae harboring the hcfc1a^co60/+ allele (Co60/+) at 5 days post fertilization (DPF) stained with Elavl3 (HuC/D) antibodies and Hoescht DNA content dye. 20× representatives of the entire brain (a, b) and 63× sub-regions (a′, b′) are shown. Wildtype siblings (N = 6) and hcfc1a^co60/+ larvae (N = 6). Arrowheads indicate regions of increased expression/localization of protein. Arrowheads of Hoescht (Nuclear) images demonstrate regions with cell bodies. Top schematic demonstrates a 5 DPF larval brain with a line to demonstrate the region depicted below. c Quantitative real time PCR analysis of the expression of elavl3, gfap, and olig2 in siblings and hcfc1a^co60/+ larvae (N = 8/group/biological replicate). Error bars represent standard error of the mean. *p < 0.05

Expression Data

Genes:	elavl3 gfap olig2
Fish:	WT hcfc1a^co60/+
Anatomical Terms:	brain brain glial cell brain neuron brain radial glial cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hcfc1a^co60/+
Observed In:	brain brain glial cell brain neuron
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.