FIGURE

Fig. 2

ID

ZDB-FIG-200416-5

Publication

Holowiecki et al., 2020 - Pbx4 limits heart size and fosters arch artery formation through partitioning second heart field progenitors and restricting proliferation

Other Figures

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Fig. 2

Cardiac fusion and elongation are abnormal in lzr mutants. (A-F) ISH for vmhc in WT and lzr mutant hearts at the 20 s stage, 24 hpf and 30 hpf. Views are dorsal with anterior up. Black arrows in A and B indicate the location of cardiac fusion when forming the cone. White arrows in B indicate anterior aggregates of CMs. Arrow in D indicates the larger ventricular pole. Arrows in F indicate already visible ventricular protrusions. At least 48 embryos per developmental stage were examined and genotyped. Scale bar: 100 µm. (G) RT-qPCR for vmhc expression in WT and Pbx4-depleted embryos at 20 s and in WT and lzr mutants at 36 hpf and 48 hpf. Error bars indicate s.e.m. *P<0.05.

Expression Data

Gene:	myh7
Fish:	WT pbx4^b557/b557 WT + MO2-pbx4 + MO3-pbx4
Knockdown Reagents:	MO2-pbx4 MO3-pbx4
Anatomical Terms:	heart rudiment presumptive cardiac ventricle heart tube whole organism
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pbx4^b557/b557
Observed In:	cardiac ventricle whole organism
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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