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Fig. 2 Cardiac fusion and elongation are abnormal in lzr mutants. (A-F) ISH for vmhc in WT and lzr mutant hearts at the 20?s stage, 24?hpf and 30?hpf. Views are dorsal with anterior up. Black arrows in A and B indicate the location of cardiac fusion when forming the cone. White arrows in B indicate anterior aggregates of CMs. Arrow in D indicates the larger ventricular pole. Arrows in F indicate already visible ventricular protrusions. At least 48 embryos per developmental stage were examined and genotyped. Scale bar: 100?µm. (G) RT-qPCR for vmhc expression in WT and Pbx4-depleted embryos at 20?s and in WT and lzr mutants at 36?hpf and 48?hpf. Error bars indicate s.e.m. *P<0.05.