FIGURE

Fig. 5

ID
ZDB-FIG-200325-197
Publication
Bagwell et al., 2020 - Notochord vacuoles absorb compressive bone growth during zebrafish spine formation
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Fig. 5

Dstyk regulates fusion of pre-vacuolar carriers with the notochord vacuole.

( A–B) Maximum projection of a light sheet microscopy live image of WT and spzl-/- embryos at 48hpf and 65hpf, respectively. ( C–D) Still images from a 17 hr light sheet movie of a WT and spzl-/-embryos zebrafish embryo during vacuole biogenesis depicting vacuole fusion events. Vacuole membranes are labeled by NFATC:gal4;UAS:GFPrab32 expression. Arrows point to GFP-Rab32 positive vesicles. Arrowheads point to large vesicle (vacuolinos) docking events. n = 2 animals per genotype. Asterisks mark fusion events. Scale bar = 100 µm.






Expression Data
Gene:
Fish:
Anatomical Term:
Stage Range: Long-pec to Pec-fin

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Long-pec to Pec-fin

Phenotype Detail
Acknowledgments
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