| ZFIN ID: ZDB-FIG-200325-202 |
Bagwell et al., 2020
- Notochord vacuoles absorb compressive bone growth during zebrafish spine formation.
eLIFE
9
Full text @ Elife
EXPRESSION / LABELING:
| Gene: | |
|---|---|
| Fish: | |
| Anatomical Term: | |
| Stage: | Days 14-20 |
PHENOTYPE:
| Fish: | |
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| Observed In: | |
| Stage: | Days 14-20 |
Fig. 8 Nuclear distribution and shape indicate that hydrostatic pressure of the notochord is decreased in spzl mutants.( A–B) Confocal images of cross sections of 14 dpf WT and spzl-/- larvae section. Vacuolated cells are labeled with SAG:gal4;UAS:GFP and nuclei are stained with DAPI. Scale bar = 50 µm ( C) Plot of vacuolated cell nuclear distribution for WT (red, n = 29) and spzl-/- (black, n = 32). ( D) Nuclear volume in WT and spzl-/- at 14 dpf. ( E–F) Reconstructions of nuclei in WT ( E) and spzl-/- ( F) and two different viewing angles. Scale bar = 20 µm ( G) Sphericity of WT and spzl-/- nuclei at 14 dpf. p-values were determined by an un-paired t-test using Welch’s correction. |
Gene Expression Details
| Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
|---|---|---|---|---|---|---|---|
| EGFP | nksagff214aGt ; nkuasgfp1aTg | standard conditions | Days 14-20 | notochord inner cell cytoplasm | IFL | ||
| Days 14-20 | Not Detected | notochord inner cell vacuolar lumen | IFL | ||||
| nksagff214aGt ; nkuasgfp1aTg ; dstyks739/s739 | standard conditions | Days 14-20 | notochord inner cell cytoplasm | IFL | |||
| Days 14-20 | Not Detected | notochord inner cell vacuolar lumen | IFL |
Antibody Labeling Details
No data available
Phenotype Details
| Acknowledgments: | ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife |
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