ZFIN ID: ZDB-FIG-200319-7
Marín-Juez et al., 2019 - Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation. Developmental Cell   51:503-515.e4 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 3 Epicardial Hypoxia Stimulates Epicardial cxcl12b Expression and Coronary EC Proliferation via Hif1a (A) qPCR analysis of aldh1a2 and egln3 mRNA levels in sorted tcf21:RFP+ cells from 7 dps and 7 dpci ventricles (n = 4). (B and C) 7 dps (B) and 7 dpci (C) ventricles stained for epicardium (green), hypoxyprobe (red), and DNA (blue). Insets show high-magnification images of sham (B) and regenerating (C) epicardium. (D) qPCR analysis of cxcl12b mRNA levels in WT ventricles at 7 dps (n = 4) and 7 dpci (n = 4) and in hif1a−/− ventricles at 7 dpci (n = 4). (E and F) WT (E) and hif1a−/− (F) ventricles stained for endothelial nuclei (red), PCNA (green), and DNA (blue). Insets show high-magnification images of proliferating cECs (white arrowheads). (G–I) cEC proliferation in WT (n = 4), and hif1a−/− (n = 4) ventricles at 96 hpci (G). Ventricles from PBS- (H) and DMOG- (I) treated animals stained for endothelial nuclei (red), PCNA (green), and DNA (blue). Insets show high-magnification images of proliferating cECs (white arrowheads). (J) cEC proliferation in PBS- (n = 4), and DMOG- (n = 4) treated ventricles at 96 hpci. White dotted lines delineate the injured area. Data in graphs expressed as mean ± SEM. ns, no significant difference, ∗p < 0.05, ∗∗p < 0.01. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 pd37Tg cryoablation: heart Adult regenerating tissue epicardium RTPCR
cxcl12b WT cryoablation: heart Adult regenerating tissue cardiac ventricle RTPCR
hif1abbns90/bns90 ; hif1aabns89/bns89 cryoablation: heart Adult regenerating tissue cardiac ventricle RTPCR
egln3 pd37Tg cryoablation: heart Adult regenerating tissue epicardium RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT cryoablation: heart Adult regenerating tissue cardiac ventricle cxcl12b expression increased amount, abnormal
WT cryoablation: heart, chemical treatment by injection: dimethyloxalylglycine Adult blood vessel endothelial cell cell population proliferation increased occurrence, abnormal
Adult regenerating tissue cell population proliferation increased occurrence, abnormal
hif1abbns90/bns90 ; hif1aabns89/bns89 cryoablation: heart Adult blood vessel endothelial cell cell population proliferation decreased occurrence, abnormal
Adult regenerating tissue cardiac ventricle cxcl12b expression amount, ameliorated
Adult regenerating tissue cell population proliferation decreased occurrence, abnormal
pd37Tg cryoablation: heart Adult regenerating tissue epicardium aldh1a2 expression increased amount, abnormal
Adult regenerating tissue epicardium egln3 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 51, Marín-Juez, R., El-Sammak, H., Helker, C.S.M., Kamezaki, A., Mullapuli, S.T., Bibli, S.I., Foglia, M.J., Fleming, I., Poss, K.D., Stainier, D.Y.R., Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation, 503-515.e4, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell