ZFIN ID: ZDB-FIG-200319-8
Marín-Juez et al., 2019 - Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation. Developmental Cell   51:503-515.e4 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 4 Vegfaa Signaling Directs Intra-ventricular Coronary Sprouting (A) 7 dpci ventricle stained for endothelium (green), Aldh1a2 (red), and DNA (blue). High-magnification images show intra-ventricular sprouting coronaries (EGFP+, Aldh1a2−) (white arrowhead) in close proximity to activated endocardium (EGFP+, Aldh1a2+) (n = 4). (B) 7 dpci ventricle stained for regenerating coronaries (green), Aldh1a2 (white), CMs (red), and DNA (blue). Regenerating superficial coronaries (yellow arrowheads). High-magnification images show a cross section of regenerating intra-ventricular coronaries surrounded by activated endocardium (n = 6) (red arrowheads). (C–E) 15 hpci (C and D) (n = 6) and 30 dpci (E) (n = 5) ventricles stained for vegfaa:EGFP expression (green), endocardium (white), CMs (red), activated endocardium ([D], white), and DNA (blue). High-magnification images of vegfaa:EGFP+ border zone endocardium (Ci; yellow arrowheads), endocardium distal to the injury (Cii), and vegfaa:EGFP+ activated endocardium (D and E; white arrowheads). (F and G) WT (F) and flt1−/− (G) ventricles stained for cardiac endothelium (green) and cECs (red). High-magnification images show intra-ventricular sprouting coronaries (white arrowheads). (H and I) Number (H) and length (I) of intra-ventricular coronaries in WT (n = 6) and flt1−/− (n = 4) animals at 96 hpci. White dotted lines delineate injured area. Data in graphs expressed as mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bars: 100 μm. See also Figure S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine mu104Tg cryoablation: heart Adult regenerating tissue coronary artery IHC
EGFP ci1Tg; hu5333Tg cryoablation: heart Adult regenerating tissue blood vessel endothelium IHC
flt1bns29/bns29; ci1Tg; hu5333Tg cryoablation: heart Adult regenerating tissue blood vessel endothelium IHC
is5Tg; pd260Tg cryoablation: heart Adult regenerating tissue endocardium IHC
y1Tg cryoablation: heart Adult regenerating tissue coronary artery IHC
Adult regenerating tissue endocardium IHC
RFP ci1Tg; hu5333Tg cryoablation: heart Adult regenerating tissue coronary artery IHC
flt1bns29/bns29; ci1Tg; hu5333Tg cryoablation: heart Adult regenerating tissue coronary artery IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-aldh1a2 IHC mu104Tg cryoablation: heart Adult regenerating tissue endocardium
IHC y1Tg cryoablation: heart Adult regenerating tissue endocardium
Phenotype Details
Fish Conditions Stage Phenotype
flt1bns29/bns29; ci1Tg; hu5333Tg cryoablation: heart Adult regenerating tissue coronary artery increased amount, abnormal
Adult regenerating tissue coronary artery increased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 51, Marín-Juez, R., El-Sammak, H., Helker, C.S.M., Kamezaki, A., Mullapuli, S.T., Bibli, S.I., Foglia, M.J., Fleming, I., Poss, K.D., Stainier, D.Y.R., Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation, 503-515.e4, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell