ZFIN ID: ZDB-FIG-200319-6
Marín-Juez et al., 2019 - Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation. Developmental Cell   51:503-515.e4 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 2 Epicardial Chemokine Expression Directs Superficial Coronary Revascularization (A–D) Ventricles at 10 dpci stained for epicardium (white), endothelium (green), CMs (red), and DNA (blue). High-magnification images of intra-ventricular sprouting coronaries (Ai, Bi; EGFP+ yellow arrowheads) and superficial coronaries (Aii, Bii; EGFP+, magenta arrowheads) in DMSO- (A) and MTZ- (B) treated animals. Number of superficial cECs (C) and intra-ventricular coronaries (D) in DMSO- (n = 6) and MTZ- (n = 6) treated animals at 10 dpci. (E) 7 dpci ventricle stained for cxcl12b:YFP expression (white), coronary ECs (green), CMs (red), and DNA (blue). High-magnification images of regenerating epicardium upregulating cxcl12b:YFP expression. (F) qPCR analysis of aldh1a2 and cxcl12b mRNA levels in sorted tcf21:RFP+ cells from 7 days post-sham (dps) and 7 dpci ventricles (n = 4). (G–K) 7 dpci ventricle stained for cxcl12b:YFP expression (green), activated epicardium and endocardium (white), CMs (red), and DNA (blue) (G). High-magnification images of regenerating cxcl12b:YFP+ epicardium (yellow arrowheads) and activated endocardium negative for cxcl12b:YFP expression (magenta arrowheads). Wholemount images of Tg(fli1a:EGFP) WT (H) and cxcr4a−/− (I) ventricles at 7 dpci. (I) High-magnification image shows an intra-ventricular sprouting coronary in a cxcr4a−/− heart (white arrowhead). Number of superficial cECs (J) and intra-ventricular coronaries (K) in WT (n = 6) and cxcr4a−/− (n = 6) animals at 7 dpci. Data in graphs expressed as mean ± SEM. ∗∗p < 0.01. Scale bars: 100 μm. See also Figures S4 and S5; Table S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 pd37Tg cryoablation: heart Adult regenerating tissue epicardium RTPCR
Citrine hu5333Tg; mu105Tg cryoablation: heart Adult regenerating tissue epicardium IHC
mu105Tg cryoablation: heart Adult regenerating tissue epicardium IHC
cxcl12b pd37Tg cryoablation: heart Adult regenerating tissue epicardium RTPCR
EGFP ci1Tg; pd108Tg cryoablation: heart, chemical ablation: epicardium, chemical treatment by environment: metronidazole Adult regenerating tissue epicardium IHC
mCherry ci1Tg; pd108Tg cryoablation: heart, chemical ablation: epicardium, chemical treatment by environment: metronidazole Adult regenerating tissue vascular endothelium IHC
RFP hu5333Tg; mu105Tg cryoablation: heart Adult regenerating tissue coronary artery IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-aldh1a2 IHC mu105Tg cryoablation: heart Adult regenerating tissue epicardium
Phenotype Details
Fish Conditions Stage Phenotype
ci1Tg; pd108Tg cryoablation: heart, chemical ablation: epicardium, chemical treatment by environment: metronidazole Adult regenerating tissue blood vessel endothelial cell decreased amount, abnormal
Adult regenerating tissue coronary artery decreased amount, abnormal
cxcr4aum20/um20; y1Tg cryoablation: heart Adult regenerating tissue blood vessel endothelial cell decreased amount, abnormal
Adult regenerating tissue coronary artery decreased amount, abnormal
pd37Tg cryoablation: heart Adult regenerating tissue epicardium aldh1a2 expression increased amount, abnormal
Adult regenerating tissue epicardium cxcl12b expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 51, Marín-Juez, R., El-Sammak, H., Helker, C.S.M., Kamezaki, A., Mullapuli, S.T., Bibli, S.I., Foglia, M.J., Fleming, I., Poss, K.D., Stainier, D.Y.R., Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation, 503-515.e4, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell