ZFIN ID: ZDB-FIG-200121-9
Yang et al., 2019 - RNA 5-Methylcytosine Facilitates the Maternal-to-Zygotic Transition by Preventing Maternal mRNA Decay. Molecular Cell   75(6):1188-1202.e11 Full text @ Mol. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Oblong to 10-13 somites

Fig. S3

Generation and Characterization of Ybx1 Mutant Zebrafish and Phenotypes of Ybx1 MO-injected Embryos. Related to Figure 3. (A) In situ hybridization results revealing the expression profile of ybx1 mRNA. (B) RNA-Seq results revealing the expression profile of ybx1 mRNA. The expression levels were normalized by the RPKM of gapdh in the corresponding stages. (C) Graphical representation of the target site in the fifth exon of ybx1 used for the design of mutants through a CRISPR/Cas9 strategy. The lower panel shows the 2 bp insertion in the ybx1 target site. (D) Whole mount in situ hybridization analysis results showing the lack of expression of ybx1 in the ybx1 mutant. (E and F) Western blotting (E) and immunofluorescence (F) results showing that Ybx1 protein was undetectable in the homozygous mutants. -Actin served as a protein loading control. Scale bar: 200 m. (G) Design of the ybx1 MO that targeted the 5’UTR (UTR MO) or the start codon (ATG MO) of ybx1 mRNA.


Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ybx1 AB standard conditions 1-cell blastomere ISH
4-cell blastomere ISH
64-cell blastomere ISH
1k-cell blastodisc ISH
Sphere blastodisc ISH
Shield blastoderm ISH
75%-epiboly blastoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-ybx1 standard conditions Oblong gastrulation delayed, abnormal
30%-epiboly gastrulation delayed, abnormal
75%-epiboly gastrulation delayed, abnormal
Bud gastrulation increased duration, abnormal
Bud somitogenesis delayed, abnormal
10-13 somites gastrulation increased duration, abnormal
10-13 somites somitogenesis delayed, abnormal
AB + MO4-ybx1 standard conditions Oblong gastrulation delayed, abnormal
30%-epiboly gastrulation delayed, abnormal
75%-epiboly gastrulation delayed, abnormal
Bud gastrulation increased duration, abnormal
Bud somitogenesis delayed, abnormal
10-13 somites gastrulation increased duration, abnormal
10-13 somites somitogenesis delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Cell