ZFIN ID: ZDB-FIG-200121-8
Yang et al., 2019 - RNA 5-Methylcytosine Facilitates the Maternal-to-Zygotic Transition by Preventing Maternal mRNA Decay. Molecular Cell   75(6):1188-1202.e11 Full text @ Mol. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 8-cell to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 1k-cell to 90%-epiboly

Fig. 3

Ybx1 Is Involved in Zebrafish Early Embryogenesis

(A) Immunofluorescence showing the protein levels of Ybx1 at the indicated stages. Scale bar, 200 μm.

(B) Time-matched images of embryos showing that the ybx1 MZ mutant experiences an early-stage delay maintained throughout early development.

(C) The fold changes in gene expression levels during MZT in control and ybx1 morphants as detected through RNA-seq. p values were calculated using two-sided Wilcoxon and Mann-Whitney tests.

(D and E) Zebrafish embryos and histograms displaying the rescue effect after coinjection of the ybx1 UTR MO (D) or the ATG MO (E) and different ybx1 mRNAs at the blastula or gastrula period. Shown are representative images of embryos (left) and histograms (right) displaying the rescue effect after coinjection of ybx1 MOs and mismatched wild-type (WT, mis-mRNA) or mutated (W45A, mis-A mRNA; W45F, mis-F mRNA) ybx1 mRNAs at the indicated time points. The numbers on the x axis and to the right of each bar indicate the embryo count with the same morphological characteristics as the presented image and the total number of embryos used for the experiment, respectively.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ybx1 AB standard conditions 8-cell blastomere IHC
128-cell blastodisc IHC
1k-cell blastodisc IHC
256-cell blastodisc IHC
Sphere blastodisc IHC
75%-epiboly blastoderm IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-ybx1 standard conditions 1k-cell gastrulation delayed, abnormal
90%-epiboly gastrulation delayed, abnormal
AB + MO4-ybx1 standard conditions 1k-cell gastrulation delayed, abnormal
90%-epiboly gastrulation delayed, abnormal
ybx1ioz107/ioz107 (AB) standard conditions Shield gastrulation arrested, abnormal
ybx1ioz107/ioz107 (AB) standard conditions Shield gastrulation delayed, abnormal
ybx1ioz107/ioz107 (AB) standard conditions 75%-epiboly whole organism viability, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Cell