ZFIN ID: ZDB-FIG-200116-3
Rissone et al., 2019 - A model for reticular dysgenesis shows impaired sensory organ development and hair cell regeneration linked to cellular stress. Disease models & mechanisms   12(12) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 3.

Characterization of PLL neuromast markers in lateral line of ak2hg14 mutants. (A,B) WISH analysis of atoh1a marker expression in primary and secondary neuromasts on 5 dpf ak2hg14 (A) and ak2hg16 (B) embryos and their siblings (WT). (C) eya1 expression from 2.5 to 5 dpf in ak2hg14 embryos and their siblings. Red arrowheads indicate the primII. Each panel shows higher magnification of the regions in dashed rectangles from corresponding pictures in Fig. S5. Scale bars: 100 µm. (D) Confocal analysis from 2 to 5 dpf of trunk regions of ak2hg14 embryos and their siblings with different transgenic backgrounds to visualize components of the lateral line rosettes. Scale bars: 50 µm. Yellow arrows indicate pou4f3:GFP+ HCs in secondary neuromasts.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh1a WT control Day 5 posterior lateral line neuromast ISH
ak2hg14/hg14 control Day 5 posterior lateral line neuromast ISH
ak2hg16/hg16 control Day 5 posterior lateral line neuromast ISH
EGFP ak2hg14/hg14; tnks1bp1y229Gt; nns8Tg control Long-pec posterior lateral line neuromast IFL
Protruding-mouth Not Detected posterior lateral line neuromast IFL
Day 4 Not Detected posterior lateral line neuromast IFL
Day 5 Not Detected posterior lateral line neuromast IFL
tnks1bp1y229Gt; nns8Tg control Long-pec posterior lateral line neuromast IFL
Protruding-mouth posterior lateral line neuromast IFL
Day 4 posterior lateral line neuromast IFL
Day 5 posterior lateral line neuromast IFL
eya1 WT control Pec-fin posterior lateral line neuromast ISH
Protruding-mouth posterior lateral line neuromast ISH
Day 4 posterior lateral line neuromast ISH
Day 5 posterior lateral line neuromast ISH
ak2hg14/hg14 control Pec-fin posterior lateral line neuromast ISH
Protruding-mouth posterior lateral line neuromast ISH
Day 4 posterior lateral line neuromast ISH
Day 5 posterior lateral line neuromast ISH
GFP ak2hg14/hg14; s356tTg control Long-pec posterior lateral line neuromast hair cell IFL
Protruding-mouth posterior lateral line neuromast hair cell IFL
Day 4 Not Detected posterior lateral line neuromast IFL
Day 5 Not Detected posterior lateral line neuromast IFL
s356tTg control Long-pec posterior lateral line neuromast hair cell IFL
Protruding-mouth posterior lateral line neuromast hair cell IFL
Day 4 posterior lateral line neuromast hair cell IFL
Day 5 posterior lateral line neuromast hair cell IFL
Tomato ak2hg14/hg14; tnks1bp1y229Gt; nns8Tg control Long-pec posterior lateral line neuromast IFL
Protruding-mouth Not Detected posterior lateral line neuromast IFL
Day 4 Not Detected posterior lateral line neuromast IFL
Day 5 Not Detected posterior lateral line neuromast IFL
tnks1bp1y229Gt; nns8Tg control Long-pec posterior lateral line neuromast IFL
Protruding-mouth posterior lateral line neuromast IFL
Day 4 posterior lateral line neuromast IFL
Day 5 posterior lateral line neuromast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ak2hg14/hg14 control Protruding-mouth posterior lateral line neuromast eya1 expression decreased amount, abnormal
Day 4 posterior lateral line neuromast eya1 expression decreased amount, abnormal
Day 5 posterior lateral line neuromast atoh1a expression decreased amount, abnormal
Day 5 posterior lateral line neuromast eya1 expression decreased amount, abnormal
ak2hg14/hg14; s356tTg control Day 4 posterior lateral line neuromast GFP expression absent, abnormal
Day 5 posterior lateral line neuromast GFP expression absent, abnormal
ak2hg14/hg14; tnks1bp1y229Gt; nns8Tg control Protruding-mouth posterior lateral line neuromast EGFP expression absent, abnormal
Protruding-mouth posterior lateral line neuromast Tomato expression absent, abnormal
Day 4 posterior lateral line neuromast EGFP expression absent, abnormal
Day 4 posterior lateral line neuromast Tomato expression absent, abnormal
Day 5 posterior lateral line neuromast EGFP expression absent, abnormal
Day 5 posterior lateral line neuromast Tomato expression absent, abnormal
Acknowledgments:
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