ZFIN ID: ZDB-FIG-200116-4
Rissone et al., 2019 - A model for reticular dysgenesis shows impaired sensory organ development and hair cell regeneration linked to cellular stress. Disease models & mechanisms   12(12) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4.

Analysis of lateral line phenotypes using different ak2 mutant transgenic lines. (A) Confocal analysis at different developmental stages of the trunk regions of ak2hg15 embryos and their control siblings (WT) in the Tg(-8.0cldnb:LY-EGFP) background. (B) Confocal analysis from 3.5 to 4 dpf of trunk regions of an ak2hg15 embryo and a control embryo in the Tg(-8.0cldnb:LY-EGFP) background labeling the migrating secondary primordium (dashed ellipse), deposited secondary neuromast (red circle), connecting interneuromast cells and epithelial cells. Confocal images were collected before and right after the end of the time-lapse confocal movie recording. (C) Confocal analysis from 3 to 5 dpf of trunk regions of ak2hg14 embryos and their siblings in different lateral line transgenic backgrounds. Yellow asterisks label the interneuromast cells between L1 and L2 in the sqet20Et transgenic line. White and yellow arrowheads denote primI or primII interneuromast cells, respectively. White asterisks label the migrating secondary primordium. Scale bars: 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP ak2hg14/hg14; sqet20Et control Protruding-mouth posterior lateral line IFL
Day 4 posterior lateral line neuromast IFL
Day 5 posterior lateral line neuromast IFL
ak2hg15/hg15; zf106Tg control Protruding-mouth posterior lateral line IFL
Protruding-mouth Not Detected posterior lateral line neuromast IFL
Day 4 posterior lateral line IFL
Day 4 Not Detected posterior lateral line neuromast IFL
Day 5 posterior lateral line IFL
Day 5 Not Detected posterior lateral line neuromast IFL
sqet20Et control Protruding-mouth posterior lateral line interneuromast cell IFL
Day 4 posterior lateral line interneuromast cell IFL
Day 5 posterior lateral line interneuromast cell IFL
zf106Tg control Protruding-mouth posterior lateral line neuromast IFL
Day 4 posterior lateral line neuromast IFL
Day 5 posterior lateral line neuromast IFL
GFP ak2hg14/hg14; sorbs2bmn0002Gt; s356tTg control Protruding-mouth posterior lateral line IFL
Day 4 posterior lateral line neuromast IFL
Day 5 posterior lateral line neuromast IFL
sorbs2bmn0002Gt; s356tTg control Protruding-mouth posterior lateral line neuromast hair cell IFL
Day 4 posterior lateral line neuromast hair cell IFL
Day 5 posterior lateral line neuromast hair cell IFL
mRFP1 ak2hg14/hg14; sorbs2bmn0002Gt; s356tTg control Protruding-mouth posterior lateral line IFL
Day 4 posterior lateral line neuromast IFL
Day 5 posterior lateral line neuromast IFL
sorbs2bmn0002Gt; s356tTg control Protruding-mouth posterior lateral line neuromast neuromast support cell IFL
Day 4 posterior lateral line neuromast neuromast support cell IFL
Day 5 posterior lateral line neuromast neuromast support cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ak2hg14/hg14; sorbs2bmn0002Gt; s356tTg control Day 4 posterior lateral line neuromast GFP expression decreased amount, abnormal
Day 4 posterior lateral line neuromast mRFP1 expression decreased amount, abnormal
Day 5 posterior lateral line neuromast GFP expression decreased amount, abnormal
Day 5 posterior lateral line neuromast mRFP1 expression decreased amount, abnormal
ak2hg14/hg14; sqet20Et control Day 4 posterior lateral line neuromast EGFP expression decreased amount, abnormal
Day 5 posterior lateral line neuromast EGFP expression decreased amount, abnormal
ak2hg15/hg15; zf106Tg control Protruding-mouth posterior lateral line neuromast EGFP expression absent, abnormal
Protruding-mouth posterior lateral line neuromast primordium migration process quality, normal
Day 4 posterior lateral line neuromast EGFP expression absent, abnormal
Day 4 posterior lateral line neuromast primordium migration process quality, normal
Day 5 posterior lateral line neuromast EGFP expression absent, abnormal
Acknowledgments:
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