FIGURE

Fig. 1.

ID

ZDB-FIG-200116-1

Publication

Rissone et al., 2019 - A model for reticular dysgenesis shows impaired sensory organ development and hair cell regeneration linked to cellular stress

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Fig. 1.

ak2 larval expression in the otic vesicle and sensory phenotypes of the zebrafish ak2 alleles. (A) WISH on wild-type zebrafish larvae at different developmental stages. Lateral (3-5 dpf) and dorsal (5 dpf) views. Black arrows point to ak2 expression in the otic vesicles. Scale bars: 200 μm. (B) Survival rates for wild-type, ak2 heterozygous, and ak2 mutant larvae at different dpf. Top and bottom graphs show survival of the progeny from an incross of ak2^hg16/+ or ak2^hg14/+ heterozygous animals, respectively. Dashed lines indicate the expected ratio following Mendelian inheritance. Numbers above the bars indicate the total number of embryos used for genotyping analysis at each stage. (C,D) Comparison of confocal maximum projections of inner ear regions (dashed circles) from Tg(pou4f3:GAP-GFP) wild type (WT) and ak2^hg14 mutants at 3 (C) and 4 (D) dpf. Scale bars: 20 µm. For each set of images, graphs show the comparison of the average number of GFP⁺ hair cells in the anterior macula (3 dpf) and cristae (3 and 4 dpf) between ak2-deficient animals and controls (WT). a, anterior; ac, anterior crista; am, anterior macula; HCs, hair cells; m, medial; mc, medial crista; NM, neuromast; p, posterior; pc, posterior crista. The error bars indicate s.d. ****P<0.0001 (two-tailed unpaired Student's test).

Expression Data

Genes:	ak2 GFP
Fish:	WT s356tTg ak2^hg14/hg14; s356tTg
Anatomical Terms:	anterior crista hair cell hair cell anterior macula lateral crista hair cell otic vesicle posterior crista hair cell
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ak2^hg14/hg14 ak2^hg16/hg16 ak2^hg14/hg14; s356tTg
Observed In:	auditory receptor cell hair cell anterior macula whole organism
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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