Figure 6
- ID
- ZDB-FIG-191230-907
- Publication
- Baron et al., 2019 - Cell Type Purification by Single-Cell Transcriptome-Trained Sorting
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GateID Allows Enrichment of α and β Cells from Human Pancreatic Islets (A) t-SNE map of the complete pancreas dataset (all pancreas TDs and unstained enrichment experiment datasets, n = 10,176 cells). Single cells are colored based on cell type. (B) Left panel: PCA of human pancreas TD2 (unstained, BD FACSJazz). Each point represents a single cell, and single cells are colored based on cell type identification from scRNA-seq. The ellipses represent normal contour lines that contain 50% of the data points for each cell type. Right panel: PC1 and PC2 loadings. Each point represents a FACS channel measured by the BD FACSJazz. (C) Contour plots of unstained human pancreas cells showing experimental gates used to sort α cells from donor 4. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated. (D) t-SNE map of human pancreas TD2, where α cells inside and outside of GateID gates are colored red and blue, respectively. (E) Contour plots of unstained human pancreas cells showing experimental gates used to sort β cells from donor 3. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated. (F) t-SNE map of human pancreas TD2, where β cells inside and outside of GateID gates are colored red and blue, respectively. (G and H) Barplots and t-SNE maps showing the outcome of GateID α and β cell enrichments for (G) donor and (H) donor 4 on BD FACSJazz. Gates were predicted on unstained TD2. See also |
Reprinted from Cell, 179, Baron, C.S., Barve, A., Muraro, M.J., van der Linden, R., Dharmadhikari, G., Lyubimova, A., de Koning, E.J.P., van Oudenaarden, A., Cell Type Purification by Single-Cell Transcriptome-Trained Sorting, 527-542.e19, Copyright (2019) with permission from Elsevier. Full text @ Cell