FIGURE

Figure S6

ID

ZDB-FIG-191230-906

Publication

Baron et al., 2019 - Cell Type Purification by Single-Cell Transcriptome-Trained Sorting

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Figure S6

Gates for Enrichments of α and β Cells from Unstained Pancreatic Tissue on BD FACSJazz, Related to Figure 6

(A) t-SNE map of human pancreas TD1 generated on on BD FACSJazz. Single cells are colored based on cell type.

(B) GateID predicted gates to isolate alpha cells from human pancreas. Gates were predicted on TD1. Red points show desired cells (alpha cells) present in TD and the blue points show undesired cells falling in the other gate.

(C) Contour plots of unstained human pancreas cells showing experimental gates used to sort alpha cells from donor 1. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated.

(D) GateID predicted gates to isolate beta cells from human pancreas. Gates were predicted on TD1. Red points show desired cells (beta cells) present in TD and the blue points show undesired cells falling in the other gate.

(E) Contour plots of unstained human pancreas cells showing experimental gates used to sort beta cells from donor 1. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated.

(F) t-SNE map of human pancreas full dataset. Single cells are colored based on experiment number.

(G) Barplots and t-SNE map showing the outcome of GateID alpha and beta cell enrichments for donor 1 on BD FACSJazz. Gates for were predicted on unstained TD1.

(H) t-SNE map of human pancreas TD2 generated on BD FACSJazz. Single cells are colored based on cell type.

(I) Barplots and t-SNE map showing the outcome of GateID beta cell enrichment for donor 2 on BD FACSJazz. Gates for were predicted on unstained TD2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 179, Baron, C.S., Barve, A., Muraro, M.J., van der Linden, R., Dharmadhikari, G., Lyubimova, A., de Koning, E.J.P., van Oudenaarden, A., Cell Type Purification by Single-Cell Transcriptome-Trained Sorting, 527-542.e19, Copyright (2019) with permission from Elsevier. Full text @ Cell