FIGURE

FIGURE 2

ID

ZDB-FIG-191230-343

Publication

Xue et al., 2019 - Zebrafish Vestigial Like Family Member 4b Is Required for Valvulogenesis Through Sequestration of Transcription Factor Myocyte Enhancer Factor 2c

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FIGURE 2

Deficiency of zebrafish vgll4b leads to impaired valvulogenesis. (A–D) WISH analysis of cmcl2 expression in zebrafish embryos at 22, 36, 48, and 96 hpf, a defective S shaped heart looping was clearly observed from 36 hpf. White dashed lines indicate the heart morphology outlined by cmlc2 expression. Lateral view, (C). (E–K) WISH analysis of vmhc, amhc, anf at 48 hpf, as well as bmp4, notch1b, klf2a, spp1 at 52 hpf. (L,M) Optical Heartbeat analysis of cardiac function. Comparison of the heart rate at 48 hpf (L) and ventricular shortening fraction at 52∼54 hfp (M). (N,O) Representative single confocal z-section images showing the endocardial cushion cells expressing Alcam (asterisks) of wild type sibling and vgll4b^–/– embryos at 52 hpf. Scale bar: 10 μm.

Expression Data

Genes:	bmp4 klf2a notch1b nppa spp1
Fish:	WT vgll4b^{zf3192/zf3192}
Anatomical Terms:	atrioventricular canal endocardial cushion atrioventricular canal myocardium atrioventricular canal endocardium atrium cardiac ventricle
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vgll4b^{zf3192/zf3192}
Observed In:	atrioventricular canal endocardium atrioventricular canal endocardium cell cardiac ventricle heart contraction
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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