Marcksb interacts with Hsp70.3 to regulate the dorsoventral patterning and the extracellular level of Bmp2b.
(A) Morphological phenotypes of embryos at 12 hpf, injected with sub-dosages of morpholinos against marcksb or hsp70 or the combination of both (marcksb_MO: 3 ng/embryo; hsp70_MO: 2 ng/embryo). (B-E) WISH analysis of szl (B), ved (C), otx2 (D), foxi1 (E). The percentage of embryos with different phenotypes for each group were indicated in the graph. Dosages of morpholino per embryo are indicated in the figure panel. For szl, ved and otx2, embryos are animal-pole view with dorsal to the right; for foxi1, embryos are lateral view with dorsal to the right. “n” represents the number of embryos we observed. (F) Quantitative measurement of secreted Bmp2b. The data were presented as scatter plots with median; “*”: P < 0.01, from Student’s t-test.