ZFIN ID: ZDB-FIG-191230-1468
Ye et al., 2019 - Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLoS Genetics   15:e1008306 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to 5-9 somites

Fig 8 Marcksb interacts with Hsp70.3 to regulate the dorsoventral patterning and the extracellular level of Bmp2b.

(A) Morphological phenotypes of embryos at 12 hpf, injected with sub-dosages of morpholinos against marcksb or hsp70 or the combination of both (marcksb_MO: 3 ng/embryo; hsp70_MO: 2 ng/embryo). (B-E) WISH analysis of szl (B), ved (C), otx2 (D), foxi1 (E). The percentage of embryos with different phenotypes for each group were indicated in the graph. Dosages of morpholino per embryo are indicated in the figure panel. For szl, ved and otx2, embryos are animal-pole view with dorsal to the right; for foxi1, embryos are lateral view with dorsal to the right. “n” represents the number of embryos we observed. (F) Quantitative measurement of secreted Bmp2b. The data were presented as scatter plots with median; “*”: P < 0.01, from Student’s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxi1 AB standard conditions 75%-epiboly non neural ectoderm ISH
AB + MO1-hsp70.3 standard conditions 75%-epiboly non neural ectoderm ISH
AB + MO1-hsp70.3 + MO1-marcksb standard conditions 75%-epiboly non neural ectoderm ISH
AB + MO1-marcksb standard conditions 75%-epiboly non neural ectoderm ISH
otx2b AB standard conditions 75%-epiboly neuroectoderm ISH
AB + MO1-hsp70.3 standard conditions 75%-epiboly neuroectoderm ISH
AB + MO1-hsp70.3 + MO1-marcksb standard conditions 75%-epiboly neuroectoderm ISH
AB + MO1-marcksb standard conditions 75%-epiboly neuroectoderm ISH
szl AB standard conditions Shield whole organism ventral region ISH
AB + MO1-hsp70.3 standard conditions Shield whole organism ventral region ISH
AB + MO1-hsp70.3 + MO1-marcksb standard conditions Shield whole organism ventral region ISH
AB + MO1-marcksb standard conditions Shield whole organism ventral region ISH
ved AB standard conditions Shield whole organism ventral region ISH
AB + MO1-hsp70.3 standard conditions Shield whole organism ventral region ISH
AB + MO1-hsp70.3 + MO1-marcksb standard conditions Shield whole organism ventral region ISH
AB + MO1-marcksb standard conditions Shield whole organism ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-hsp70.3 standard conditions Shield whole organism ventral region szl expression decreased amount, abnormal
Shield whole organism ventral region szl expression decreased distribution, abnormal
Shield whole organism ventral region ved expression decreased amount, abnormal
Shield whole organism ventral region ved expression decreased distribution, abnormal
75%-epiboly neuroectoderm otx2b expression increased amount, abnormal
75%-epiboly neuroectoderm otx2b expression increased distribution, abnormal
75%-epiboly non neural ectoderm foxi1 expression decreased amount, abnormal
75%-epiboly non neural ectoderm foxi1 expression decreased distribution, abnormal
AB + MO1-hsp70.3 + MO1-marcksb standard conditions Shield whole organism ventral region szl expression decreased amount, abnormal
Shield whole organism ventral region szl expression decreased distribution, abnormal
Shield whole organism ventral region ved expression decreased amount, abnormal
Shield whole organism ventral region ved expression decreased distribution, abnormal
75%-epiboly neuroectoderm otx2b expression increased amount, abnormal
75%-epiboly neuroectoderm otx2b expression increased distribution, abnormal
75%-epiboly non neural ectoderm foxi1 expression decreased amount, abnormal
75%-epiboly non neural ectoderm foxi1 expression decreased distribution, abnormal
5-9 somites whole organism spindle-shaped, abnormal
AB + MO1-marcksb standard conditions Shield whole organism ventral region szl expression decreased amount, abnormal
Shield whole organism ventral region szl expression decreased distribution, abnormal
Shield whole organism ventral region ved expression decreased amount, abnormal
Shield whole organism ventral region ved expression decreased distribution, abnormal
75%-epiboly neuroectoderm otx2b expression increased amount, abnormal
75%-epiboly neuroectoderm otx2b expression increased distribution, abnormal
75%-epiboly non neural ectoderm foxi1 expression decreased amount, abnormal
75%-epiboly non neural ectoderm foxi1 expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.