ZFIN ID: ZDB-FIG-191230-1465
Ye et al., 2019 - Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLoS Genetics   15:e1008306 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 2-cell to Shield
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 2-cell to Prim-15

Fig 5 MZ<italic>marcksb</italic> do not show visible dorsoventral defects.

(A) The diagram shows the gRNA target of marcksb genome locus and the genotypes of two individual marcksb mutants generated by CRISPR/Cas9. (B) The transcription level of marcksb was significantly decreased in MZmarcksb embryos. Embryos of 2-cell stage, high stage and shield stage were present. All the embryos were lateral view with animal-pole to the top. (C) Both MZmarcksb and marcksb morphants showed similar defect of epiboly movement. The blue bar indicates the distance between the inner layer of cells and the envelope layer. (D) The wildtype and MZmarcksb embryos at developmental stages of 0.75 hpf, 3.5 hpf, 7 hpf, 12 hpf and 35hpf. MZmarcksb showed yolk bulge phenotype during early somite stage but normal appearance of development at 35 hpf. “Arrow” indicates the extrusion of the vegetal-most part of the yolk.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
marcksb AB standard conditions 2-cell whole organism ISH
High whole organism ISH
Shield whole organism ISH
marcksbihb199/ihb199 standard conditions 2-cell whole organism ISH
High whole organism ISH
Shield whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-marcksb standard conditions Shield - 75%-epiboly epiboly involved in gastrulation with mouth forming second process quality, abnormal
marcksbihb199/ihb199 standard conditions 2-cell whole organism marcksb expression decreased amount, abnormal
High whole organism marcksb expression decreased amount, abnormal
Shield epiboly involved in gastrulation with mouth forming second process quality, abnormal
Shield whole organism marcksb expression decreased amount, abnormal
Shield - 75%-epiboly epiboly involved in gastrulation with mouth forming second process quality, abnormal
5-9 somites yolk protruding out of yolk, abnormal
Prim-15 whole organism morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.