FIGURE

Fig 5

ID

ZDB-FIG-191230-1465

Publication

Ye et al., 2019 - Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b

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Fig 5

MZ<italic>marcksb</italic> do not show visible dorsoventral defects.

(A) The diagram shows the gRNA target of marcksb genome locus and the genotypes of two individual marcksb mutants generated by CRISPR/Cas9. (B) The transcription level of marcksb was significantly decreased in MZmarcksb embryos. Embryos of 2-cell stage, high stage and shield stage were present. All the embryos were lateral view with animal-pole to the top. (C) Both MZmarcksb and marcksb morphants showed similar defect of epiboly movement. The blue bar indicates the distance between the inner layer of cells and the envelope layer. (D) The wildtype and MZmarcksb embryos at developmental stages of 0.75 hpf, 3.5 hpf, 7 hpf, 12 hpf and 35hpf. MZmarcksb showed yolk bulge phenotype during early somite stage but normal appearance of development at 35 hpf. “Arrow” indicates the extrusion of the vegetal-most part of the yolk.

Expression Data

Gene:	marcksb
Fish:	AB marcksb^{ihb199/ihb199}
Anatomical Term:	whole organism
Stage Range:	2-cell to Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	marcksb^{ihb199/ihb199} AB + MO1-marcksb
Knockdown Reagent:	MO1-marcksb
Observed In:	epiboly involved in gastrulation with mouth forming second whole organism yolk
Stage Range:	2-cell to Prim-15

Phenotype Detail

Acknowledgments

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