ZFIN ID: ZDB-FIG-191230-1461
Ye et al., 2019 - Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLoS Genetics   15:e1008306 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to Prim-5

Fig 1 Marcksb is required for specification of ventral cell fate.

(A) Knockdown of marcksb showed dorsalization defects, which could be rescued by overexpression of morpholino insensitive mRNA of marcksb. Up panels, field view of embryos at early-somite stage; lower panels, representative embryos at 24 hours post fertilization (hpf). (B) The percentage of embryos with normal-like or dorsalization defects. “n” represents the number of embryos we observed. (C-F) Whole-mount in situ hybridization (WISH) showed the expansion of dorsal markers otx2 (neural ectoderm) (C) and chordin (dorsal margin) (D) expression, and the shrinkage of ventral markers foxi1 (non-neural ectoderm) (E) and eve1 (ventral margin) (F) expression in marcksb morphants. The percentage of embryos with indicated phenotype were shown aside their representative images. For otx2, foxi1 and eve1, embryos are lateral view with animal-pole to the top and dorsal to the right; for chd, embryos are animal-pole view with dorsal to the right; arrows in chd panels indicate the expansion locations of signals. (G) A schematic showing the procedure of the tail organizer transplantation assay. (H) A representative wildtype-to-wildtype transplantation embryo showing an induction of ectopic tail by grafting the tail organizer region of the wildtype embryo. (I) A representative morphant-to-wildtype transplantation embryo without induction of ectopic tissue by grafting the tail organizer region of the marcksb morphant embryo. The ratio at the right corner indicates the number of embryos with the representative phenotype/the total number of observed embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrd AB standard conditions Shield shield ISH
AB + MO1-marcksb standard conditions Shield shield ISH
eve1 AB standard conditions 75%-epiboly margin ventral region ISH
AB + MO1-marcksb standard conditions 75%-epiboly margin ventral region ISH
foxi1 AB standard conditions 75%-epiboly non neural ectoderm ISH
AB + MO1-marcksb standard conditions 75%-epiboly non neural ectoderm ISH
otx2b AB standard conditions 75%-epiboly neuroectoderm ISH
AB + MO1-marcksb standard conditions 75%-epiboly neuroectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-marcksb standard conditions Shield shield chrd expression increased distribution, abnormal
75%-epiboly margin ventral region eve1 expression decreased distribution, abnormal
75%-epiboly neuroectoderm otx2b expression increased distribution, abnormal
75%-epiboly non neural ectoderm foxi1 expression decreased distribution, abnormal
1-4 somites - 5-9 somites whole organism spindle-shaped, abnormal
Prim-5 whole organism dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.