ZFIN ID: ZDB-FIG-191220-10
Yang et al., 2019 - TAMM41 is required for heart valve differentiation via regulation of PINK-PARK2 dependent mitophagy. Cell death and differentiation   26(11):2430-2446 Full text @ Cell Death Differ.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7

Enforced mitochondrial fission couldn’t restore heart valve malformation with tamm41 mutant embryos. a qPCR analysis of mitophagy related gene expression (dnm1l, park2, fundc1, mfn1b) in tamm41+/+ and tamm41−/− zebrafish embryos. b Representative images reveal the failure of MO-mfn1b injection to recover the abnormally expressed heart valve markers in tamm41−/− embryos (lower panels). Red arrows indicate the normally expressed heart valve genes. c, d Representative images of three independent experiments revealed that elevated mitochondrial membrane proteins in tamm41−/− embryos could not be restored with MO-mfn1b injection. e, f DNM1L-GFP overexpression failed to decrease the elevated mitochondrial membrane proteins in si-TAMM41 AC16 cells with CCCP treatment (fourth panels). g, h Representative images (n = 50 cells) show that DNM1L-GFP overexpression is incapable of restoring defective mitophagy in TAMM41 deficient AC16 cells with CCCP addition. Scale bar: 5 μm. Black horizontal lines indicate mean ± SD. Means ± SD are shown for three independent experiments. n.s, not significant, *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp4 WT standard conditions Long-pec atrioventricular canal ISH
WT + MO1-mfn1b standard conditions Long-pec atrioventricular canal ISH
tamm41rj109/rj109 standard conditions Long-pec heart ISH
tamm41rj109/rj109 + MO1-mfn1b standard conditions Long-pec heart ISH
dnm1l tamm41rj109/rj109 standard conditions Protruding-mouth whole organism RTPCR
fundc1 tamm41rj109/rj109 standard conditions Protruding-mouth whole organism RTPCR
mfn1b tamm41rj109/rj109 standard conditions Protruding-mouth whole organism RTPCR
notch1b WT standard conditions Long-pec atrioventricular canal ISH
WT + MO1-mfn1b standard conditions Long-pec atrioventricular canal ISH
tamm41rj109/rj109 standard conditions Long-pec heart ISH
tamm41rj109/rj109 + MO1-mfn1b standard conditions Long-pec heart ISH
prkn tamm41rj109/rj109 standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tamm41rj109/rj109 standard conditions Long-pec heart bmp4 expression increased distribution, abnormal
Long-pec heart notch1b expression increased distribution, abnormal
Protruding-mouth whole organism dnm1l expression increased amount, abnormal
Protruding-mouth whole organism fundc1 expression increased amount, abnormal
Protruding-mouth whole organism mfn1b expression increased amount, abnormal
Protruding-mouth whole organism prkn expression increased amount, abnormal
tamm41rj109/rj109 + MO1-mfn1b standard conditions Long-pec heart bmp4 expression increased distribution, abnormal
Long-pec heart notch1b expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell death and differentiation for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Differ.