Fig. S1
- ID
- ZDB-FIG-191126-12
- Publication
- Hsu et al., 2019 - Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration
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MicroRNA screen experimental design. (A) Each of the miR candidate gene is cloned in the intron of a green fluorescence reporter, driven by a neutrophil specific lyzC promoter. The plasmid is injected into 1-cell stage zebrafish embryos to induce expression of miR in a subset of neutrophils. (B) A representative picture of a bacterial infection which induces neutrophil recruitment to the otic vesicle. (C) Decreased neutrophil recruitment due to the overexpression miR-199-3. An inhibition of 50% neutrophil infiltrating into the ear indicates a positive hit |