FIGURE

Fig. S8

ID

ZDB-FIG-191126-18

Publication

Hsu et al., 2019 - Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration

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Fig. S8

WT Cdk2 partially rescues neutrophil motility defects resulted from miR-199 overexpression.

(A) Schematics of experimental design. Embryos from Tg(lyzC:miR-199-Dendra2)pu19 were injected with a plasmid driving miR-199-resistant cdk2 expression in neutrophils, Tol2-lyzC-mCherry-2a-Cdk2 WT . Single (GFP+, miR-199 only) and double positive cells (GFP+mCherry+, miR-199+cdk2+) from the same embryos were tracked. (B, C) Representative images and tracks (B), and quantification of velocity (C) single and double positive cells. Scale bar: 100 μm. Green tracks: miR-199 expression only; Red tracks: miR-199 and cdk2 WT expression. 3 embryos each from three different founders were individual injected, imaged and quantified. Results are presented as mean ± s.d., Mann–Whitney test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA