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Fig. S1
MicroRNA screen experimental design.
(A) Each of the miR candidate gene is cloned in the intron of a green fluorescence reporter, driven by a neutrophil specific lyzC promoter. The plasmid is injected into 1-cell stage zebrafish embryos to induce expression of miR in a subset of neutrophils. (B) A representative picture of a bacterial infection which induces neutrophil recruitment to the otic vesicle. (C) Decreased neutrophil recruitment due to the overexpression miR-199-3. An inhibition of 50% neutrophil infiltrating into the ear indicates a positive hit
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Full text @ Proc. Natl. Acad. Sci. USA