FIGURE

Fig. S4

ID

ZDB-FIG-191126-14

Publication

Hsu et al., 2019 - Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration

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Fig. S4

The CDK2 inhibitor CTV313 or a pan cdk inhibitor roscovitine reduces neutrophil migration.

(A, B) Representative images (A) and quantification (B) of neutrophils recruited to infected ear in zebrafish larva treated with a cdk2 inhibitor (CTV313) or a pan-CDK inhibitor (roscovitine). Scale bar: 100 μm. (C, D) Representative images (C) and quantification (D) of neutrophils recruited to tail fin transection sites in zebrafish larva treated with CTV313 or roscovitine. Scale bar: 200 μm. (E, F) Representative images (E) and quantification (F) of total neutrophil number in zebrafish larva treated with CTV313 or roscovitine. Scale bar: 500 μm. (A-F) Assays were done with at least 3 individual founders with 3 biological repeats each containing 20 (for motility) or 25 (for neutrophil recruitment) fish per group. Result is presented as mean ± s.d., Mann–Whitney test. (G, H) Representative images (G), and quantification of velocity (H) of neutrophils in zebrafish larvae treated with CTV313 or Roscovitine. Scale bar: 100 μm. 3 embryos each from three different founders were imaged and quantification of neutrophils in one representative movie is shown, Kruskal–Wallis test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA