FIGURE

Fig. 3

ID

ZDB-FIG-190923-10

Publication

Chambers et al., 2019 - Tfap2a is a novel gatekeeper of nephron differentiation during kidney development

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Fig. 3

tfap2a and tfap2b function redundantly to activate distal nephron solute transporter signature. (A) Whole-mount in situ hybridization for slc12a1 (DE, purple), slc12a3 (DL, red) and clcnk (pan-distal, purple) at 24 hpf. Black bars indicate wild-type marker domains. Black dots encircle cysts in the duct; black arrowhead indicates incomplete fusion of cloaca. Scale bar: 35 µm. (B-D) Absolute length quantifications of (B) slc12a1, (C) slc12a3 and (D) clcnk. n≥3. Measurements compared by ANOVA. Data are mean±s.d. *P<0.05; **P<0.01; ***P<0.001; N.S., not significant.

Expression Data

Genes:	clcnk slc12a1 slc12a3
Fish:	TU tfap2a^nt30/nt30 tfap2b^{sa10090/sa10090} TU + MO1-tfap2b TU + MO4-tfap2a tfap2a^nt30/nt30 + MO1-tfap2b tfap2b^{sa10090/sa10090} + MO4-tfap2a TU + MO1-tfap2b + MO4-tfap2a
Knockdown Reagents:	MO1-tfap2b MO4-tfap2a
Anatomical Terms:	pronephric distal early tubule pronephric distal late tubule pronephros distal region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tfap2a^nt30/nt30 tfap2b^{sa10090/sa10090} TU + MO1-tfap2b TU + MO4-tfap2a tfap2a^nt30/nt30 + MO1-tfap2b tfap2b^{sa10090/sa10090} + MO4-tfap2a TU + MO1-tfap2b + MO4-tfap2a
Knockdown Reagents:	MO1-tfap2b MO4-tfap2a
Observed In:	posterior pronephric duct pronephric distal early tubule pronephric distal early tubule early distal convoluted tubule development pronephric distal early tubule pronephric nephron tubule epithelial cell differentiation pronephric distal late tubule pronephric distal late tubule pronephric nephron tubule epithelial cell differentiation pronephros distal region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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