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Fig. 3 tfap2a and tfap2b function redundantly to activate distal nephron solute transporter signature. (A) Whole-mount in situ hybridization for slc12a1 (DE, purple), slc12a3 (DL, red) and clcnk (pan-distal, purple) at 24 hpf. Black bars indicate wild-type marker domains. Black dots encircle cysts in the duct; black arrowhead indicates incomplete fusion of cloaca. Scale bar: 35 µm. (B-D) Absolute length quantifications of (B) slc12a1, (C) slc12a3 and (D) clcnk. n≥3. Measurements compared by ANOVA. Data are mean±s.d. *P<0.05; **P<0.01; ***P<0.001; N.S., not significant.
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