FIGURE

Fig. 10-S1

ID
ZDB-FIG-190718-32
Publication
Pujala et al., 2019 - Chronology-based architecture of descending circuits that underlie the development of locomotor repertoire after birth
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Fig. 10-S1

Confirmation of hindbrain V2a neuron ablations at four dpf.

(A) Maximum intensity projections of hindbrain V2a neurons expressing Kaede from a fish in which early-born neurons were ablated (see Figure 11B i-ii) as well as from a control fish at four dpf (left, dorsal view; right, side view; magenta, photoconverted Kaede; green, unconverted Kaede). Contrast of magenta channel was enhanced to improve the visibility of early-born neurons, which made late-born neurons appear white. Open arrowheads indicate locations of early-born neurons (magenta). Note the lack of magenta cells in the ablated fish. Asterisks mark the bright structures in the red channel located outside the brain. r, rostral; c, caudal; d, dorsal; v ventral; scale bars, 30 μm. (B) Maximum intensity projections of hindbrain V2a neurons from a fish in which dorsocaudal late-born neurons were ablated (see Figure 11B iii-iv) as well as a control fish at four dpf (left, dorsal view; right, side view; magenta, photoconverted Kaede; green, unconverted Kaede). Rounded dotted rectangle in each image indicates the location of neurons that were targeted in the ablated fish. r, rostral; c, caudal; d, dorsal; v ventral; scale bars, 30 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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