FIGURE

Fig. 5

ID

ZDB-FIG-190329-5

Publication

Pouchucq et al., 2018 - γ-Tubulin small complex formation is essential for early zebrafish embryogenesis

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Fig. 5

Cell proliferation arrest and apoptosis triggered in the absence of γ-TuSC formation. A. Zebrafish control and morphant embryos treated through the BrdU incorporation procedure. Embryos (8 hpf) were exposed to a BrdU pulse for 1 h, then fixed and treated for DNA labeling with DAPI (red) and immunolabeled for the incorporated BrdU (green). tubg1-Mo embryos did not incorporate BrdU in their DNA, indicating proliferation arrest. B. Apoptotic response revealed by TUNEL assay in embryos (8 hpf). The tubg1-morphant showed many clusters of cells positive for TUNEL stain (arrowheads) distributed throughout the blastoderm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tubg1
Knockdown Reagent:	MO1-tubg1
Observed In:	blastomere apoptotic process blastomere cell population proliferation
Stage:	75%-epiboly

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.