|ZFIN ID: ZDB-FIG-190329-5|
Cell proliferation arrest and apoptosis triggered in the absence of γ-TuSC formation. A. Zebrafish control and morphant embryos treated through the BrdU incorporation procedure. Embryos (8 hpf) were exposed to a BrdU pulse for 1 h, then fixed and treated for DNA labeling with DAPI (red) and immunolabeled for the incorporated BrdU (green). tubg1-Mo embryos did not incorporate BrdU in their DNA, indicating proliferation arrest. B. Apoptotic response revealed by TUNEL assay in embryos (8 hpf). The tubg1-morphant showed many clusters of cells positive for TUNEL stain (arrowheads) distributed throughout the blastoderm.
|WT + MO1-tubg1||standard conditions||75%-epiboly||blastomere apoptotic process increased occurrence, abnormal|
|75%-epiboly||blastomere cell population proliferation arrested, abnormal|
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Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.