ZFIN ID: ZDB-FIG-190329-5
Pouchucq et al., 2018 - γ-Tubulin small complex formation is essential for early zebrafish embryogenesis. Mechanisms of Development   154:145-152 Full text @ Mech. Dev.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 75%-epiboly

Fig. 5

Cell proliferation arrest and apoptosis triggered in the absence of γ-TuSC formation. A. Zebrafish control and morphant embryos treated through the BrdU incorporation procedure. Embryos (8 hpf) were exposed to a BrdU pulse for 1 h, then fixed and treated for DNA labeling with DAPI (red) and immunolabeled for the incorporated BrdU (green). tubg1-Mo embryos did not incorporate BrdU in their DNA, indicating proliferation arrest. B. Apoptotic response revealed by TUNEL assay in embryos (8 hpf). The tubg1-morphant showed many clusters of cells positive for TUNEL stain (arrowheads) distributed throughout the blastoderm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tubg1 standard conditions 75%-epiboly blastomere apoptotic process increased occurrence, abnormal
75%-epiboly blastomere cell population proliferation arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.