ZFIN ID: ZDB-FIG-190329-2
Pouchucq et al., 2018 - γ-Tubulin small complex formation is essential for early zebrafish embryogenesis. Mechanisms of Development   154:145-152 Full text @ Mech. Dev.
Anatomical Terms:
Stage Range: 4-cell to 75%-epiboly

Fig. 1

Maternal-zygotic zebrafish γ-tubulin expression and subcellular localization during early development. A. RT-PCR analysis revealed by electrophoresis in agarose gel showed that γ-tubulin mRNA is detected during the first 8 h of the zebrafish embryogenesis. B. qPCR analysis indicated that γ-tubulin mRNA expression levels were invariant during the first 8 h of zebrafish embryogenesis. β-actin and eef1a were used as reference genes. C. Immunoblot analysis showed that γ-tubulin protein is present during the cleavage (1–3 hpf) and post-MBT stages (3–8 hpf) of zebrafish development. Actin was used as a loading control. D–E. Fluorescence microscopy images of zebrafish embryos (2 hpf) double-stained with anti-γ-tubulin antibody (green) and DAPI (red). Metaphase (E), anaphase, and telophase (F) stages of the cell cycle are shown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tubg1 WT standard conditions 4-cell whole organism RTPCR
64-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tubg1 IHC WT standard conditions 64-cell blastomere centrosome
IHC 64-cell blastomere perinuclear region of cytoplasm
WB 4-cell whole organism
WB 64-cell whole organism
WB 1k-cell whole organism
WB 30%-epiboly whole organism
WB Sphere whole organism
WB Shield whole organism
WB 75%-epiboly whole organism
Phenotype Details No data available
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Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.