|ZFIN ID: ZDB-FIG-190329-2|
|Stage Range:||4-cell to 75%-epiboly|
Maternal-zygotic zebrafish γ-tubulin expression and subcellular localization during early development. A. RT-PCR analysis revealed by electrophoresis in agarose gel showed that γ-tubulin mRNA is detected during the first 8 h of the zebrafish embryogenesis. B. qPCR analysis indicated that γ-tubulin mRNA expression levels were invariant during the first 8 h of zebrafish embryogenesis. β-actin and eef1a were used as reference genes. C. Immunoblot analysis showed that γ-tubulin protein is present during the cleavage (1–3 hpf) and post-MBT stages (3–8 hpf) of zebrafish development. Actin was used as a loading control. D–E. Fluorescence microscopy images of zebrafish embryos (2 hpf) double-stained with anti-γ-tubulin antibody (green) and DAPI (red). Metaphase (E), anaphase, and telophase (F) stages of the cell cycle are shown.
|tubg1||WT||standard conditions||4-cell||whole organism||RTPCR|
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article.
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Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.