ZFIN ID: ZDB-FIG-190329-4
Pouchucq et al., 2018 - γ-Tubulin small complex formation is essential for early zebrafish embryogenesis. Mechanisms of Development   154:145-152 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 64-cell to Sphere
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Sphere to 75%-epiboly

Fig. 4

Nuclear and microtubule organization defects in γ-tubulin morphant embryos. A–D. Fluorescence microscopy images of control and morphant embryos at 2 (A and C) and 4 (B and D) hpf, showing centrosomal (γ-tubulin, green) and chromosomal (DAPI, red) distribution. E–H. anti-α-tubulin antibodies (green) and DAPI (red) double-stained embryos at 2 (E and G) and 4 (F and H) hpf. In the morphant embryos, γ-tubulin and microtubule organizations were severely affected and γ-TuSC formation impairment generated multinucleated blastomeres (arrowheads in D and H). I, J. Confocal projection images of α-tubulin-immunofluorescence staining of blastoderm in 8 hpf control and morphant embryos. Microtubule-stained spindle structures (arrowheads in I) and bundle organizations are shown. N, nucleus. K, L. DAPI-stained nuclei of control and tubg1-Mo embryos show a severe karyokinesis defect after γ-tubulin silencing.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tubg1 IHC WT standard conditions 64-cell blastomere centrosome
IHC Sphere blastomere centrosome
IHC WT + MO1-tubg1 standard conditions 64-cell blastomere centrosome
IHC Sphere blastomere cytoplasm
IHC Sphere blastomere perinuclear region of cytoplasm
Ab2-tuba IHC WT standard conditions 64-cell blastomere microtubule
IHC Sphere blastomere microtubule
IHC WT + MO1-tubg1 standard conditions 64-cell blastomere microtubule
IHC Sphere blastomere microtubule
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tubg1 standard conditions Sphere blastomere cytoplasm ab1-tubg1 labeling mislocalised, abnormal
Sphere blastomere has extra parts of type blastomere nucleus, abnormal
Sphere blastomere perinuclear region of cytoplasm ab1-tubg1 labeling mislocalised, abnormal
Sphere cell cycle process quality, abnormal
Sphere microtubule cytoskeleton organization process quality, abnormal
75%-epiboly blastomere has extra parts of type blastomere nucleus, abnormal
75%-epiboly cell cycle process quality, abnormal
75%-epiboly microtubule cytoskeleton organization process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.