|ZFIN ID: ZDB-FIG-190329-3|
|Stage Range:||75%-epiboly to Prim-5|
γ-tubulin silencing by morpholino-based knockdown causes developmental arrest during gastrulation. A. Bright field images of the representative phenotypes obtained in a tubg1-Mo (20 nM) injection experiment and complete rescue phenotype. Control injected embryos did not show a phenotype. Images were taken at 8 and 24 hourspost-fertilization (hpf). The frequency of each phenotypic class (severe, moderate and wild type) is indicated as a percentage. The standard deviations correspond to three independent biological replicas. B. Phenotypes distribution of 24 hpf embryos injected with tubg1-Mo (20 and 10 nM), control Mo (20 nM) and tubg1-Mo (20 mM) + γ-tubulin polypeptide.
|WT + MO1-tubg1||standard conditions||75%-epiboly||embryo development arrested, abnormal|
|Prim-5||embryo development arrested, abnormal|
|Prim-5||whole organism morphology, abnormal|
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Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.