ZFIN ID: ZDB-FIG-190329-3
Pouchucq et al., 2018 - γ-Tubulin small complex formation is essential for early zebrafish embryogenesis. Mechanisms of Development   154:145-152 Full text @ Mech. Dev.
Knockdown Reagent:
Observed In:
Stage Range: 75%-epiboly to Prim-5

Fig. 2

γ-tubulin silencing by morpholino-based knockdown causes developmental arrest during gastrulation. A. Bright field images of the representative phenotypes obtained in a tubg1-Mo (20 nM) injection experiment and complete rescue phenotype. Control injected embryos did not show a phenotype. Images were taken at 8 and 24 hourspost-fertilization (hpf). The frequency of each phenotypic class (severe, moderate and wild type) is indicated as a percentage. The standard deviations correspond to three independent biological replicas. B. Phenotypes distribution of 24 hpf embryos injected with tubg1-Mo (20 and 10 nM), control Mo (20 nM) and tubg1-Mo (20 mM) + γ-tubulin polypeptide.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tubg1 standard conditions 75%-epiboly embryo development arrested, abnormal
Prim-5 embryo development arrested, abnormal
Prim-5 whole organism morphology, abnormal
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.