FIGURE

Fig. 4

ID

ZDB-FIG-190131-15

Publication

Gibb et al., 2018 - Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development

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Fig. 4

Expression of hey2 in CPCs during cardiac development. (A-L′) Double RNA in situ hybridization analysis of hey2 (A,B,D-F,H-J,L,L′) and Tg(epicon21:EGFP) expression (C,G,K) as compared with nkx2.5 (A,H,H′), myl7 (C-E,G,I,K-L′) and mef2cb (B,F,J) in wild-type embryos at 16.5 hpf (A-D), 20 hpf (E-H′) and 24 hpf (I-L′). The arrowheads in D',E-G indicate hey2⁺ cells outside of the cardiac (myl7⁺) region. (M-N″) Immunofluorescence showing Tg(epicon21:EGFP) enhancer expression when compared with Tg(nkx2.5:ZsYellow) at 20 hpf (M-M″) and Tg(myl7:mCherry-RAS) at 24 hpf (N-N″). (O) Schematic representation of hey2 and myl7 progenitor localization and movement patterns from 16.5 to 24 hpf. Scale bars: 50 µm. Asterisk labels pharyngeal mesoderm. Arrows indicate the atrium (N-N″) and arrowheads mark the ventricle (I-L′,N-N′).

Expression Data

Genes:	EGFP hey2 mCherry mef2cb myl7 nkx2.5 ZsYellow
Fish:	AB/TL hsc28Tg fb7Tg; hsc28Tg hsc28Tg; sd21Tg
Anatomical Terms:	anterior lateral plate mesoderm heart primordium heart rudiment mesoderm pharyngeal mesoderm pharyngeal mesoderm anatomical region presumptive atrium heart tube presumptive cardiac ventricle heart tube primitive heart tube
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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