FIGURE

Fig. 5

ID

ZDB-FIG-190131-16

Publication

Gibb et al., 2018 - Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development

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Fig. 5

Lineage contribution of hey2⁺ cells to cardiac development. (A,B) Schematic of the experimental design for lineage tracing of hey2⁺ cells. (C-F) MF20 and EGFP immunofluorescence staining at 48 hpf showing distribution of Cre-marked cardiomyocytes. Arrowheads indicate labelled cardiomyocytes at the poles of the heart. (G) Bar graph illustrating the contribution of recombined cells to various regions of the heart following 4-HT treatment at 16.5, 19, 22 and 24 hpf (N=3, n=12 at 16.5 hpf; n=6 at 19 hpf; n=16 at 22 hpf; n=14 at 24 hpf). (H) Schematic representation showing the cardiac regions used for recombination analysis. (I-L) Representative images of Tg(epicon21:CreERT2)^hsc104×Tg(actb2:RSG) hearts at 72 hpf following 4-HT treatment at 16.5 hpf (I), 19 hpf (J), 22 hpf (K) and 24 hpf (L). Recombined cells are shown in green, with unmarked cells of the heart represented in magenta. A, atrium; V, ventricle; OFT, outflow tract. Scale bars: 50 µm.

Expression Data

Genes:	DsRed EGFP
Fish:	hsc104Tg; s928Tg
Condition:	chemical treatment by environment: afimoxifene
Anatomical Term:	cardiac ventricle
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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