FIGURE

Fig. 5

ID
ZDB-FIG-190131-16
Publication
Gibb et al., 2018 - Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development
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Fig. 5

Lineage contribution of hey2+ cells to cardiac development. (A,B) Schematic of the experimental design for lineage tracing of hey2+ cells. (C-F) MF20 and EGFP immunofluorescence staining at 48?hpf showing distribution of Cre-marked cardiomyocytes. Arrowheads indicate labelled cardiomyocytes at the poles of the heart. (G) Bar graph illustrating the contribution of recombined cells to various regions of the heart following 4-HT treatment at 16.5, 19, 22 and 24?hpf (N=3, n=12 at 16.5?hpf; n=6 at 19?hpf; n=16 at 22?hpf; n=14 at 24?hpf). (H) Schematic representation showing the cardiac regions used for recombination analysis. (I-L) Representative images of Tg(epicon21:CreERT2)hsc104×Tg(actb2:RSG) hearts at 72?hpf following 4-HT treatment at 16.5?hpf (I), 19?hpf (J), 22?hpf (K) and 24?hpf (L). Recombined cells are shown in green, with unmarked cells of the heart represented in magenta. A, atrium; V, ventricle; OFT, outflow tract. Scale bars: 50 µm.
Expression Data
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
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