FIGURE

Fig. S2

ID

ZDB-FIG-190401-4

Publication

Gibb et al., 2018 - Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Creation of a hey2 transgenic reporter line.

(A) Schematic representation of hey2 enhancer (epigenetic conserved region 21) located 24kb upstream of hey2 locus used to create Tg(epicon21:EGFP) . (B-G) Confocal images of Tg(epicon21:EGFP) and Tg(myl7:mCherry-RAS) at 48 hpf (B), 72 hpf (C, D) and 96 hpf (E, G; F with MF20). (H) Schematic methodology of internal epitope tagging of V5 into the hey2 locus with corresponding sequence of tag-specific PCR fragments. (I) Immunofluorescence of Tg(hey2-V5) internal epitope tagging with Tg(myl7:mCherry-RAS) at 30 hpf comparing Hey2 expression (green) with Myl7 (red). Scale bars 50 μm. OFT, outflow tract; V, ventricle and A, atrium; Asterisk indicates atrioventricular canal. Arrowheads mark epicon21+ OFT region (B-F) and ventricular myocardium (G).

Expression Data

Genes:	EGFP mCherry
Fish:	hsc28Tg; sd21Tg
Anatomical Terms:	bulbus arteriosus cardiac ventricle heart ventricular myocardium
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development