FIGURE

Fig. S5

ID

ZDB-FIG-190118-9

Publication

Gutzman et al., 2018 - Basal constriction during midbrain-hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase

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Fig. S5

Expression of human FAK does not rescue the laminin mutant basal constriction phenotype. (A-F’) Live confocal images showing the MHB region of prim-6 embryos injected with mGFP (A,D), mGFP + wt FAK mRNA (B,E), mGFP + FAK-Y397E (C,F). (A-C’) sleepy (slym86) (Schier et al., 1996) heterozygous sibling or wild-type sibling embryos showing normal basal constriction at the MHBC. (D-F’) sleepy mutants showing defects in basal constriction both without and with the co-injection of human wild-type FAK and human phosphomimetic FAKY397E mRNA. Representative images from 3 independent experiments with n>3 for each condition. (A’-F’) Magnifications of the neuroepithelium shown in A-F with individual cells outlined at the MHBC. Arrowheads indicate MHBC. M, midbrain. H, hindbrain. Scale bars: 35 m. Biology Open (2018): doi:10.1242/bio.034520: Supplementary information Biology Open • Supplementary information

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lamc1^m86/m86
Observed In:	midbrain hindbrain boundary constriction epithelial cell morphogenesis midbrain hindbrain boundary constriction midbrain-hindbrain boundary morphogenesis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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