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Fig. S5
Expression of human FAK does not rescue the laminin mutant basal constriction phenotype. (A-F?) Live confocal images showing the MHB region of prim-6 embryos injected with mGFP (A,D), mGFP + wt FAK mRNA (B,E), mGFP + FAK-Y397E (C,F). (A-C?) sleepy (slym86) (Schier et al., 1996) heterozygous sibling or wild-type sibling embryos showing normal basal constriction at the MHBC. (D-F?) sleepy mutants showing defects in basal constriction both without and with the co-injection of human wild-type FAK and human phosphomimetic FAKY397E mRNA. Representative images from 3 independent experiments with n>3 for each condition. (A?-F?) Magnifications of the neuroepithelium shown in A-F with individual cells outlined at the MHBC. Arrowheads indicate MHBC. M, midbrain. H, hindbrain. Scale bars: 35 ?m. Biology Open (2018): doi:10.1242/bio.034520: Supplementary information Biology Open ? Supplementary information