Fig. S3
- ID
- ZDB-FIG-181003-22
- Publication
- Yakulov et al., 2018 - CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury
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Pronephros repair is independent of EMT. In situ hybridization for genes characteristic for epithelial-to-mesenchymal transition (EMT) or de-differentiation. Two-day-old embryos were injured, fixed 4 hours after wounding, and stained for EMT-typical genes (N-myc, vimentin, snail, netrin and mmp9), or for genes characteristic for non-differentiated pronephric cells (pax2.1 and tbx2b). None of the markers showed up-regulation in the repair region, outlined by a black bracket. The staining for mmp9 and snail was performed in combination with staining for cdh17 labeling the pronephric cells in red (Scale bar, 100 μm). |